Journal: Bioactive Materials

Article Title: Natural killer cell-inspired dendritic mesoporous rare-earth nanoparticles potentiate X-ray-triggered reactive oxygen generation for low-dose radiotherapy-radiodynamic therapy

doi: 10.1016/j.bioactmat.2026.02.011

Figure Lengend Snippet: In vitro targeted cell uptake and oxygen supply ability. (a) SDS-PAGE electrophoresis profiles and Western blot results of TSSI-Ce 6 C-DMTm, NKEV, and TSSI-Ce 6 C-DMTm@NKEV. (b) Scheme of the tumor-targeting mechanism of TSSI-Ce 6 C-DMTm@NKEV. (c) Western blot results of NK cells, NKEV, TSSI-Ce 6 C-DMTm@NKEV, and TSSI-Ce 6 C-DMTm to indicate the presence of DNAM-1 and NKG2D. (d) CLSM images and quantification analysis of MDA-MB-231 cells treated with TSSI-Ce 6 C-DMTm and TSSI-Ce 6 C-DMTm@NKEV for 1, 3, and 9 h. (f) Flow cytometry profiles and (g) CLSM images of MDA-MB-231 cells pretreated with different antibodies and treated with TSSI-Ce 6 C-DMTm@NKEV for 9 h. (h) CLSM images of MDA-MB-231 cells pretreated with H 2 O 2 and treated with different formulations via [Ru(dpp) 3 ]Cl 2 staining for hypoxia levels observation. ∗∗∗∗ p < 0.0001.

Article Snippet: Then, the membrane was blocked using 5% skim milk and incubated using primary antibody of anti -DNAM-1 (ABclonal, A23200), anti -NKG2D (Bioss, bs-0938R), anti -β-actin (Beijing Solarbio Science & Technology Co., Ltd.), anti-Na/K ATPase (Abcam, ab254025), respectively.

Techniques: In Vitro, SDS Page, Electrophoresis, Western Blot, Flow Cytometry, Staining

Journal: Bioactive Materials

Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion

doi: 10.1016/j.bioactmat.2025.11.038

Figure Lengend Snippet: Preparation and characterization of ANCM@SHM. (a) Schematic diagram of NCM preparation. (b) Representative TEM images of native CM and NCM. Bar: 50 nm. (c–d) Zeta potential (n = 3), particle size (n = 3), and PDI (n = 3) of native CM and NCM measured by DLS. (e) Representative TEM images of Lipo, A-lipo, and ANCM. Bar: 50 nm. (f–g) Zeta potential (n = 3), particle size (n = 3), and PDI (n = 3) of Lipo, A-lipo, and ANCM measured by DLS. (h) Representative confocal fluorescence micrographs showing red fluorescently labeled A-lipo and green fluorescently labeled NCM fusing into gold fluorescent ANCM. Bar: 10 μm. (i) Quantification of NKG2D on the surface of CM, NCM, and ANCM nanoparticles by flow cytometry. (j–k) Encapsulation efficiency (EE) (n = 3) and loading efficiency (LE) (n = 3) of ABT263. (l) Representative SEM image of ANCM@SHM. Bar: 100 μm. (m) Representative fluorescence image of PKH67-ANCM@SHM captured by confocal microscope. Bar: 100 μm.

Article Snippet: To establish NKG2D-overexpressing chondrocytes, primary mouse chondrocytes were transfected with the full-length murine NKG2D plasmid (≈700 bp, Sangon Biotechnology, China) using Lipofectamine 3000 (Biosharp, China) according to the manufacturer's protocol.

Techniques: Zeta Potential Analyzer, Fluorescence, Labeling, Flow Cytometry, Encapsulation, Microscopy

Journal: Bioactive Materials

Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion

doi: 10.1016/j.bioactmat.2025.11.038

Figure Lengend Snippet: Targeting effects of ANCM@SHM on senescent chondrocytes. (a) Experimental schematic diagram for evaluating the ability of ANCM to target chondrocytes. (b–c) Flow cytometry images and statistical analysis representing the specific uptake of PKH67-ANCM by chondrocyte, MSC, and MC3T3 (n = 6). (d) Representative WB bands of membrane proteins in NKG2D-overexpressed chondrocyte, NCM, and ANCM nanoparticles. (e) Experimental schematic diagram for evaluating the ability of ANCM to target senescent chondrocytes. (f–g) Flow cytometry images and statistical analysis representing the specific uptake of PKH67-ANCM by senescent chondrocytes (n = 3). (h–i) Representative WB bands and quantitative analysis of the protein level of NKG2D in senescent or normal chondrocytes treated with ACM or ANCM (n = 3). (j) Representative confocal images of chondrocytes exposed to LysoTracker and FITC-labeled nanoparticles for 1, 4, or 8 h. Bar: 5 μm. (k) Representative confocal images showing the co-localization of lysosomes in senescent cells and control chondrocytes with the taken-in PKH67-labeled nanoparticles. Bar: 5 μm. (l) Representative fluorescence images indicating the adhesion and penetration of PKH67-labeled nanoparticles in articular cartilage. Bar: 50 μm. (m–n) Representative IVIS images and radiant efficiency of mice after a single intra-articular injection of DiR-labeled Lipo@SHM, EM@SHM, and ANCM@SHM within 13 days (n = 3). The data are expressed as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. Statistical significance was explored through unpaired Student's t-tests or the one-way ANOVA.

Article Snippet: To establish NKG2D-overexpressing chondrocytes, primary mouse chondrocytes were transfected with the full-length murine NKG2D plasmid (≈700 bp, Sangon Biotechnology, China) using Lipofectamine 3000 (Biosharp, China) according to the manufacturer's protocol.

Techniques: Flow Cytometry, Membrane, Labeling, Control, Fluorescence, Injection